Chromotek gfp-trap® magnetic agarose
WebGFP and Cfap91-GFP proteins were purified from the cytoplasm of GFP or Cfap91-GFP overexpressing cells using magnetic agarose beads-conjugated anti-GFP antibodies and after washings, incubated with a cytoplasmic extract obtained from cells overexpressing HA-Ccdc39 proteins (118 kDa). WebGFP-Trap™ Agarose is an affinity resin for IP of GFP-fusion proteins. Brand: Proteintech GTA-20. Additional Details : Weight : 0.00240kg. View more versions of this product. …
Chromotek gfp-trap® magnetic agarose
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WebApr 10, 2024 · Total cell extracts of yeast cells untreated or treated with CHX were incubated in ChromoTek GFP-Trap Agarose beads (Proteintech) for 2.5 h at 4 °C to pull-down AGP2-GFP tagged-associated proteins. After incubation, the beads coupled to proteins were washed with washing buffer (10 mM Tris/Cl pH 7.5, 0.5 mM EDTA, 150 … WebSep 16, 2024 · ChromoTek GFP-Trap® magnetic agarose affinity beads ( see Note 2 ). 2. Low protein binding microcentrifuge tubes (e.g., LoBind® tubes). 3. Magnetic separation rack (should fit 1.5-ml microcentrifuge tubes). 4. Table-top centrifuge (cooled, with rotors suitable for 1.5- and 2-ml microcentrifuge tubes, RCF:19,000 × g ). 5.
WebThe ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to … WebThe ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to …
WebFisher Scientific - Proteintech The ChromoTek GFP-Trap Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. Shop Proteintech ChromoTek GFP … WebMar 31, 2016 · View Full Report Card. Fawn Creek Township is located in Kansas with a population of 1,618. Fawn Creek Township is in Montgomery County. Living in Fawn …
WebSuperior results in a fraction of the time - The ChromoTek GFP-Trap® overcomes these problems: The high quality GFP-binding protein is …
WebThe ChromoTek GFP-Trap® Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. It comprises a GFP Nanobody/ VHH coupled to … foamers and bottlesWebJan 15, 2024 · ChromoTek's Nano-Traps are optimized for the immunoprecipitation (IP) of proteins and their interacting factors. Nano-Traps comprise of a Nanobody/ V H H conjugated to beads. For 3 Nano-Traps, e.g. GFP-Trap ® , RFP-Trap ®, and Spot-Trap ®, we offer different types of beads: Agarose beads Magnetic Agarose beads Magnetic … foamers animal facilitiesWebThe ChromoTek GFP-Trap™ Magnetic Agarose are affinity beads for IP of GFP-fusion proteins. Brand: Proteintech GTMA-400 Additional Details : Weight : 0.00480kg View more versions of this product Product Code. 17353363 55828.28 NOK / 400 reactions Quantity Estimated Shipment: 12-05-2024 Add to basket Description Specifications greenwich to archwayWebJun 11, 2024 · ChromoTek GFP-Trap® Magnetic Agarose beads (other commercially available affinity purification systems can be used) and a magnetic separation rack. 2.3 Elution of Proteins 1. 0.5 M Ammonium hydroxide (pH > 10): Prepare solution the day of the experiment. 200 μL of solution is needed per sample. 2. Vacuum dryer apparatus. greenwich tip opening timesWeb1. Get more from your GFP/RFP fusion Alpaca GFP-Trap ® is the top selling GFP antibody as it allows for one-step pulldowns that can be directly used in downstream applicaitons such as enzyme activity assays or mass spectrometry. 2. Best results, qualitative and quantitative GFP-/RFP trap are so easier to use than any other pull down reagent. greenwich time vs eastern timeWebApr 11, 2024 · A 500 μl fraction of each cleared sample was mixed with 50 μl of pre-equilibrated GFP-Trap magnetic agarose (ChromoTek Cat No. gtma) or DYKDDDDK Fab-Trap Agarose (ChromoTek Cat No. ffa) and incubated over-night at 4°C rotating end-over-end. The beads were then washed twice with Wash buffer (10 mM Tris–HCl pH 7.5; 150 … foamer scannerWebYou can use Chromotek GFP-trap magnetic agarose beads for the pull-down/co-immunoprecipitation assays. These magnetic beads are coupled to GFP nanobody and are ready to use. Only you need to label ... greenwich time vs london time