Ctcf zinc finger folding cristallography

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August undefined, 2024

WebSep 13, 2024 · An exceptionally conserved transcriptional repressor, CTCF, employs different combinations of zinc fingers to bind diverged promoter sequences of avian and mammalian c-myc oncogenes. Mol. Cell ... WebA characteristic feature of the CTCF is that the central part of the protein contains a cluster consisting of eleven domains of C2H2-type zinc fingers, five of which specifically bind to …

CTCF - Wikipedia

WebOct 1, 2001 · CTCF is an evolutionarily conserved zinc finger (ZF) phosphoprotein that binds through combinatorial use of its 11 ZFs to approximately 50 bp target sites that have remarkable sequence variation. WebThe sites 45 to 47 correspond to CTCF zinc fingers 1 to 3. Based on the analysis combined with Figure 4, there were significant sequence differences observed between the positive … great wall of china model project

Zinc Finger Protein CTCF Regulates Extracellular Matrix (ECM) …

WebAug 1, 2024 · A CTCF domain sgRNA library screening identifies the zinc finger (ZF) domain as the region within CTCF with the most functional relevance, including ZFs 1 and 10. WebMay 14, 2002 · The ubiquitous, highly conserved, multivalent 11-zinc-finger (ZF) factor CTCF plays multiple roles in gene regulation, depending on the combinatorial utilization of different ZFs to bind varying CTCF target sites ().CTCF–DNA complex formation can be regulated by CpG-specific DNA methylation (2, 3) if the involved CpG nucleotides are … WebJun 28, 2024 · CTCF is a multivalent 11-zinc finger DNA-binding protein that is ubiquitously expressed in most tissues of vertebrate species . Combinatorial usage of CTCF zinc fingers (4, 5) allows its binding to tens of thousands of conserved DNA sites as revealed by chromatin immunoprecipitation sequencing (ChIP-seq) experiments . More than 30% and … florida highway patrol tampa fl

CTCF and Cohesin in Genome Folding and Transcriptional Gene …

Web5.1 CTCF genome organizer and the cohesin complex. CTCF (CCCTC-binding factor) is a multiple zinc finger protein that exerts diversified functions under different genomic contexts. CTCF was first isolated and cloned on the basis of its ability to bind to highly divergent 50–60 bp sequences within the promoter region of the chicken Myc gene ... WebMar 6, 2024 · However, the role of CTCF in DNA damage response remains elusive. Here, we show that CTCF is quickly recruited to the sites of DNA damage. The fast recruitment is mediated by the zinc finger domain and poly (ADP-ribose) (PAR). Further analyses show that only three zinc finger motifs are essential for PAR recognition. florida hiking trail mapWebCTCF is an evolutionarily conserved zinc finger (ZF) phosphoprotein that binds through combinatorial use of its 11 ZFs to ∼50 bp target sites that have remarkable sequence variation. Formation of different CTCF–DNA complexes, some of which are methylation-sensitive, results in distinct functions, including gene activation, repression, silencing and … florida highway patrol lake city florida

"WebThe CTCF zinc finger domain is required for recruitment to DSB Numerousproteinsinvolved inthe DNA repairprocessare PARylated (23), and this posttranslational modifi cation can play a significant role in the recruitment of proteins to DNA breakages ( 9, 29). We observe that the association of CTCF with PARylation is substantially enriched " - Ctcf zinc finger folding cristallography

Ctcf zinc finger folding cristallography

CTCF participates in DNA damage response via poly(ADP …

WebJul 29, 2024 · The 11 zinc finger (ZF) protein CTCF regulates topologically associating domain formation and transcription through selective binding … WebSep 8, 2024 · CTCF is an 11-zinc-finger DNA-binding protein that acts as a transcriptional repressor and insulator as well as an architectural protein required for 3D genome …

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WebCTCFL, a testis-specific DNA binding protein containing 11 C2H2-type zinc fingers, belongs to the CTCF zinc-finger protein family. CTCFL is responsible for insulator … WebJul 2, 2024 · The 11 zinc fingers (ZFs) of the transcription factor CTCF play a versatile role in the regulation of gene expression. CTCF binds to numerous genomic sites to form chromatin loops and ...

WebThe endogenous CTCF migrates as a 130 kDa (CTCF-130) protein on SDS-PAGE, however, the open reading frame (ORF) of the CTCF cDNA encodes only a 82 kDa … WebMar 1, 2012 · The linker domains of the CTCF zinc finger domain were found to be phosphorylated during mitosis. The phosphorylation of linker domains impaired the …

WebHowever, the 3′ end of the CTCF motif is occupied by the N-terminal CTCF zinc fingers , and cohesin appears to bind the CTCF C terminus . Finally, CTCF may form local secondary DNA structures ( 83 ), which have been invoked to account for the directionality of loop formation between CTCF binding sites ( 102 ). WebDec 31, 2016 · The linker domains of the CTCF zinc finger domain were found to be phosphorylated during mitosis. The phosphorylation of linker domains impaired the DNA-binding activity in vitro. Mutation analyses showed that amino acid residues (Thr289, Thr317, Thr346, Thr374, Ser402, Ser461, and Thr518) located in the linker domains …

WebJun 5, 2015 · CCCTC-binding factor (CTCF) is an 82-kDa protein with 11 zinc fingers. 1 It was first identified as a transcriptional repressor of the chicken c-myc gene, a regulatory gene that encodes the c-myc ...

WebJun 1, 2024 · Recent work shows that the N-terminus of CTCF is essential for CTCF-anchored loops [25∗∗, 26∗, 27∗, 28∗∗].Using different approaches, multiple studies identified that CTCF's ability to promote looping lies between its amino acids 179–265, adjacent to CTCF's zinc fingers (Figure 1) [25, 26, 28].These findings are also in agreement with … florida highway traffic conditionsWebFeb 16, 2024 · Silencing CTCF or COL1A1/COL1A3 markedly inhibited cell growth and migration in the metastatic GC cell line BGC823. Collectively, this ECM-related 7-gene signature provides a novel insight for survival prediction among GC patients. The zinc finger protein CTCF regulates ECM-related genes, thereby promoting GC cell growth … great wall of china monterey ca great wall of china mountainsWebOct 16, 2024 · CCCTC-binding factor (CTCF) plays fundamental roles in transcriptional regulation and chromatin architecture maintenance. CTCF is also a tumour suppressor … great wall of china movie castWebJun 5, 2015 · CCCTC-binding factor (CTCF) is a highly conserved zinc finger protein and is best known as a transcription factor. It can function as a transcriptional activator, a repressor or an insulator ... great wall of china mountain rangeWebApr 1, 2024 · CTCF and CTCFL contain N-terminal and C-terminal domains and a central 11-zinc finger region. CTCF binds to approximately 80 000 sites on mammalian chromosomes [33, 34] through a subset of its eleven zinc fingers (ZFs).The ZFs of CTCFL and CTCF have significant homology with the ZFs of CTCF (74.1%), but the N-terminal … great wall of china mythsWebDNA binding by CTCF zinc fingers in human genome architecture ... genes for investigating 3D genome folding and gene reg-ulation (Supplementary information, Figure S1A) [3, 20]. great wall of china movies