Iptg isopropyl β-d-thiogalactoside
WebIsopropyl-β-D-thiogalactoside Isopropyl β- thiogalactoside (2R,3R,4S,5R,6S)-2- (hydroxymethyl)-6-propan-2-ylsulfanyloxane-3,4,5-triol CAS 367-93-1 Q: What is an operon? An operon is a functioning unit of genomic DNA that has a cluster of genes. They are under the control of a single regulatory signal or promoter. Q: What is the LAC operon? Isopropyl β-d-1-thiogalactopyranoside (IPTG) is a molecular biology reagent. This compound is a molecular mimic of allolactose, a lactose metabolite that triggers transcription of the lac operon, and it is therefore used to induce protein expression where the gene is under the control of the lac operator. See more Like allolactose, IPTG binds to the lac repressor and releases the tetrameric repressor from the lac operator in an allosteric manner, thereby allowing the transcription of genes in the lac operon, such as the gene … See more When stored as a powder at 4 °C or below, IPTG is stable for 5 years. It is significantly less stable in solution; Sigma recommends storage for no more than a month at room temperature. IPTG is an effective inducer of protein expression in the concentration range of 100 See more • IPTG bound to proteins in the PDB See more
Iptg isopropyl β-d-thiogalactoside
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Webコロナ禍によって、イソプロピル-β-D-チオガラクトシド(IPTG)（Isopropyl-β-D-Thiogalactoside (IPTG)）の世界市場規模は2024年に 百万米ドルと予測され、2029年ま … WebThe amplified fragment was subcloned into pET32a(+) or pET32b(+) and induced by isopropyl-β-d-thiogalactoside (IPTG) to express recombinant proteins in Escherichia coli …
WebIsopropyl-β-D-thiogalacto side (IPTG) is a chemical analog of galactose that cannot be cleaved by β-galactosidase. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ. Other Notes: For life science research only. WebIPTG配制 . Prepare a 1 M solution of IPTG (Isopropyl-β-D-thiogalactoside; Isopropyl-β-D-thiogalactopyranoside) by dissolving 2.38 g of IPTG in dd water and adjust the final volume to 10 ml. Filter sterilize before use. 氯霉素配制 . Chloramphenicol 34 mg/ml in ethanol. Store at -20℃. Use at 34 µg/ml. 注意事项
WebApplication. IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white … WebAmbion™ IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant …
WebIPTG (isopropyl-β-D-thiogalactopyranoside) 99% dioxane-free Supplier: Thermo Scientific Chemicals Warning Synonyms: (2R,3R,4S,5R,6S)-2- (Hydroxymethyl)-6- …
WebSep 23, 2024 · At present, isopropyl β-D-thiogalactopyranoside (IPTG) is the universal inducer for expressing recombinant proteins under the lac operator/repressor system. In this study, we propose an... sharwoods slow cooker korma sauce 170gWebIPTG. IPTG即Isopropyl β-D-1-thiogalactopyranoside，也称Isopropyl β-D-thiogalactoside，中文名为异丙基-β-D-硫代半乳糖苷。. 分子式为C9 H18 O5 S，分子量为238.30，CAS Number 367-93-1，Ultra Pure，dioxane free，纯度>99%。. 进口分装。. 本产品为接近白色的粉末。. 常用分子生物学试剂，常用 ... sharwoods mango and ginger chutneyWeb制药机械设备供应网会员企业湖北鸿鑫瑞宇精细化工有限公司提供367-93-1-IPTG诱导剂，异丙基-β-D-硫代吡喃半乳糖苷,中文品名: 异丙基-β-D-硫代吡喃半乳糖苷商品名称: Isopropyl β-D-1-Thiogalactopyranoside英文简称：IPTG产品别名: Isopropyl β-D-Thiogalactoside通用CAS porsche centre chester cheshire oaksWebMay 14, 2024 · IPTG（异丙基硫代半乳糖苷，Isopropyl β-D-Thiogalactoside)是一种作用极强的诱导剂，不被细菌代谢而十分稳定，因此被实验室广泛应用。中文品名:异丙基-β-D-硫代吡喃半乳糖苷 商品名称: Isopropyl β-D-1-Thiogalactopyranoside 英文简称: IPTG 产品别名: Isopropyl β-D-Thiogalactoside 通用CAS : sharwoods mango chutney ingredientsWebIPTG (isopropyl beta-D-thiogalactoside) Inducer for Beta-Galactosidase Expression is utilized in cloning experiments to determine positive incorporation of a construct of interest as well as in inducing recombinant protein expression. Purity/Specificity IPTG is >99.0% (enzymatic) Expand Description Specifications porsche centre doha showroomWebIsopropyl-β-D-thiogalactopyranoside: Description: Inducer of β-D-galactosidase. Used in the stimulation of β-galactosidase in cellular systems in which dioxane would disrupt normal cell function. IPTG induces the expression of cloned genes linked to the lac promoter. Form: White to almost white solid: CAS number: 367-93-1: Chemical formula ... porsche centre bristol used carsWebJan 1, 2024 · The transformed cells are spread on plates containing appropriate antibiotic (to select for the presence of plasmid, whether recombinant or empty vector), IPTG (isopropyl β-d-thiogalactoside), and X-gal. IPTG induces transcription from the lac promoter in the plasmid. Cells that acquired an empty vector in the transformation procedure … porsche centre bournemouth used cars for sale